Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27695 | 3' | -53.9 | NC_005882.1 | + | 2467 | 0.7 | 0.431573 |
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Target: 5'- uGGCGGaCgaaGCCAAAaCGAgaucgaaggggcuGGUCGAGCu -3' miRNA: 3'- gCCGCC-Ga--UGGUUUgGCU-------------UCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 30873 | 0.7 | 0.432565 |
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Target: 5'- gGGcCGGCUGCCAgugAACgGAGGUgaugucggaGAGCu -3' miRNA: 3'- gCC-GCCGAUGGU---UUGgCUUCAg--------CUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 17646 | 0.7 | 0.462924 |
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Target: 5'- aGGCgccacgGGCUGCCGAGCUGAaccuGGUUGAcuucGCc -3' miRNA: 3'- gCCG------CCGAUGGUUUGGCU----UCAGCU----CG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 19251 | 0.7 | 0.462924 |
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Target: 5'- cCGGCGGCUGCgc-ACCGAGGaaccCGAcGUg -3' miRNA: 3'- -GCCGCCGAUGguuUGGCUUCa---GCU-CG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 17351 | 0.77 | 0.160799 |
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Target: 5'- uCGGCGGCaGCUucaugcaguAGCCGAGGUCGAccGCu -3' miRNA: 3'- -GCCGCCGaUGGu--------UUGGCUUCAGCU--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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