miRNA: 3'- -GCCGC-CGAUG-GUU---------------UGGCUuCAgCUCG- -5'
miRNA: 3'- -GCCGCCGaUGGuUUGGCUu---CAGCU-CG- -5'
miRNA: 3'- gCCGCCGaug--GUUUG-GCUUCaGCUCg -5'
miRNA: 3'- -GCCGCcGA--UGGuuUgGCUUCAGCUCG- -5'
miRNA: 3'- -GCCGCCGAUGGUUUGGCUUCAGCUCG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27695 | 3' | -53.9 | NC_005882.1 | + | 14748 | 0.67 | 0.609424 |
|
Target: 5'- uCGGCGaGCUGCuCAAggccgcgauggcgcuGCUGAuGUgGGGCg -3' miRNA: 3'- -GCCGC-CGAUG-GUU---------------UGGCUuCAgCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 31971 | 0.68 | 0.571066 |
|
Target: 5'- uCGGCGGCcGCU-GACCGAuuacGUCGAcGUc -3' miRNA: 3'- -GCCGCCGaUGGuUUGGCUu---CAGCU-CG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 24090 | 0.68 | 0.571066 |
|
Target: 5'- gGGCGGCcuggaCAAGCuCGAAGgCGAGa -3' miRNA: 3'- gCCGCCGaug--GUUUG-GCUUCaGCUCg -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 32569 | 0.68 | 0.559885 |
|
Target: 5'- uCGGCGcCUagGCUuuGgCGAAGUUGAGCu -3' miRNA: 3'- -GCCGCcGA--UGGuuUgGCUUCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 29962 | 1.12 | 0.000562 |
|
Target: 5'- gCGGCGGCUACCAAACCGAAGUCGAGCu -3' miRNA: 3'- -GCCGCCGAUGGUUUGGCUUCAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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