Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27697 | 5' | -48.9 | NC_005882.1 | + | 26146 | 0.66 | 0.936645 |
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Target: 5'- ---cGGCCGCGAAGGCGccGgcaGuGGc -3' miRNA: 3'- ggaaUUGGCGCUUCCGCaaCaa-CuCC- -5' |
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| 27697 | 5' | -48.9 | NC_005882.1 | + | 10669 | 0.66 | 0.936645 |
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Target: 5'- ---cAGCgGCGGAGGCGgcggcagcgUGUUGAc- -3' miRNA: 3'- ggaaUUGgCGCUUCCGCa--------ACAACUcc -5' |
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| 27697 | 5' | -48.9 | NC_005882.1 | + | 14763 | 0.67 | 0.897752 |
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Target: 5'- ---aGGCCGCGAuGGCGcUGcugaugUGGGGc -3' miRNA: 3'- ggaaUUGGCGCUuCCGCaACa-----ACUCC- -5' |
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| 27697 | 5' | -48.9 | NC_005882.1 | + | 30074 | 0.7 | 0.778885 |
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Target: 5'- ----cACCGCGAAaggcgacgucGGCGUUGUUGGa- -3' miRNA: 3'- ggaauUGGCGCUU----------CCGCAACAACUcc -5' |
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| 27697 | 5' | -48.9 | NC_005882.1 | + | 9236 | 0.71 | 0.70129 |
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Target: 5'- gCCUg---CGCGAGGaGCGUgagcgGUUGGGGu -3' miRNA: 3'- -GGAauugGCGCUUC-CGCAa----CAACUCC- -5' |
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| 27697 | 5' | -48.9 | NC_005882.1 | + | 30836 | 1.15 | 0.00135 |
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Target: 5'- cCCUUAACCGCGAAGGCGUUGUUGAGGg -3' miRNA: 3'- -GGAAUUGGCGCUUCCGCAACAACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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