Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27699 | 5' | -53.4 | NC_005882.1 | + | 21987 | 0.66 | 0.699265 |
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Target: 5'- uGAAGU-UUGCCGUCGcGAUCgUGCUGc -3' miRNA: 3'- uCUUCGcAACGGCAGC-UUGG-ACGACu -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 12675 | 0.66 | 0.687897 |
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Target: 5'- ---cGCGaUGCCGUCGAACaaGCg-- -3' miRNA: 3'- ucuuCGCaACGGCAGCUUGgaCGacu -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 15813 | 0.67 | 0.641966 |
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Target: 5'- uGGAGCGgcGCCGcCGcGACUUGCg-- -3' miRNA: 3'- uCUUCGCaaCGGCaGC-UUGGACGacu -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 5781 | 0.67 | 0.641966 |
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Target: 5'- cAGGAGCGcgGCgGggCcGACCUGCUGc -3' miRNA: 3'- -UCUUCGCaaCGgCa-GcUUGGACGACu -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 12406 | 0.67 | 0.630428 |
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Target: 5'- cGAaccAGCGUUGCCGaUCGGcgccgccggcaGCCgucacgcGCUGAu -3' miRNA: 3'- uCU---UCGCAACGGC-AGCU-----------UGGa------CGACU- -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 23332 | 0.68 | 0.572982 |
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Target: 5'- cAGAGGCGUacGCgGUCGcugGACgUGUUGAa -3' miRNA: 3'- -UCUUCGCAa-CGgCAGC---UUGgACGACU- -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 25864 | 0.69 | 0.527915 |
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Target: 5'- aGGAAGCcg-GCCagaaaUCGAACCUGCUcGGg -3' miRNA: 3'- -UCUUCGcaaCGGc----AGCUUGGACGA-CU- -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 20598 | 0.73 | 0.29012 |
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Target: 5'- uGAAGgGcaucgUGaCCGUCG-ACCUGCUGAa -3' miRNA: 3'- uCUUCgCa----AC-GGCAGCuUGGACGACU- -5' |
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| 27699 | 5' | -53.4 | NC_005882.1 | + | 31437 | 1.09 | 0.000857 |
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Target: 5'- aAGAAGCGUUGCCGUCGAACCUGCUGAu -3' miRNA: 3'- -UCUUCGCAACGGCAGCUUGGACGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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