Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27701 | 3' | -57.3 | NC_005882.1 | + | 22767 | 0.74 | 0.140328 |
Target: 5'- cGUCC-CGCUGCCGUag-UCGUAGACCg -3' miRNA: 3'- -UAGGcGUGACGGCAaggAGCGUCUGG- -5' |
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27701 | 3' | -57.3 | NC_005882.1 | + | 26209 | 0.69 | 0.291739 |
Target: 5'- -gUCGcCACUGCCGgcgCCuUCGCGG-CCg -3' miRNA: 3'- uaGGC-GUGACGGCaa-GG-AGCGUCuGG- -5' |
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27701 | 3' | -57.3 | NC_005882.1 | + | 26640 | 0.68 | 0.364974 |
Target: 5'- -gCCGCgcgcuGCUGCCG-UCgCUCaCGGACCu -3' miRNA: 3'- uaGGCG-----UGACGGCaAG-GAGcGUCUGG- -5' |
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27701 | 3' | -57.3 | NC_005882.1 | + | 27577 | 0.67 | 0.43013 |
Target: 5'- -gCCGCGCgcgcgGCCGccggcauggCgaCGCAGGCCg -3' miRNA: 3'- uaGGCGUGa----CGGCaa-------GgaGCGUCUGG- -5' |
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27701 | 3' | -57.3 | NC_005882.1 | + | 29179 | 0.69 | 0.314801 |
Target: 5'- -gCCgGCGC-GCCGUUgCUCGCGugcGGCCg -3' miRNA: 3'- uaGG-CGUGaCGGCAAgGAGCGU---CUGG- -5' |
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27701 | 3' | -57.3 | NC_005882.1 | + | 29760 | 0.66 | 0.491078 |
Target: 5'- uUCCGCACggGCCGgcUCCaaggcaacgcUGCAGACg -3' miRNA: 3'- uAGGCGUGa-CGGCa-AGGa---------GCGUCUGg -5' |
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27701 | 3' | -57.3 | NC_005882.1 | + | 32415 | 1.1 | 0.00028 |
Target: 5'- cAUCCGCACUGCCGUUCCUCGCAGACCa -3' miRNA: 3'- -UAGGCGUGACGGCAAGGAGCGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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