Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27701 | 3' | -57.3 | NC_005882.1 | + | 26209 | 0.69 | 0.291739 |
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Target: 5'- -gUCGcCACUGCCGgcgCCuUCGCGG-CCg -3' miRNA: 3'- uaGGC-GUGACGGCaa-GG-AGCGUCuGG- -5' |
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| 27701 | 3' | -57.3 | NC_005882.1 | + | 15339 | 0.7 | 0.284351 |
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Target: 5'- -gCUGCGCaacgUGCCGUUCCggcagcaggaGCAGAUCg -3' miRNA: 3'- uaGGCGUG----ACGGCAAGGag--------CGUCUGG- -5' |
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| 27701 | 3' | -57.3 | NC_005882.1 | + | 1488 | 0.71 | 0.243111 |
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Target: 5'- uGUUCGUcaUGUCGUcCCUCGCAGugCg -3' miRNA: 3'- -UAGGCGugACGGCAaGGAGCGUCugG- -5' |
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| 27701 | 3' | -57.3 | NC_005882.1 | + | 21610 | 0.71 | 0.224423 |
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Target: 5'- -gCCGCGCUcCCGgacaCCgugUCGCAGACCu -3' miRNA: 3'- uaGGCGUGAcGGCaa--GG---AGCGUCUGG- -5' |
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| 27701 | 3' | -57.3 | NC_005882.1 | + | 22767 | 0.74 | 0.140328 |
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Target: 5'- cGUCC-CGCUGCCGUag-UCGUAGACCg -3' miRNA: 3'- -UAGGcGUGACGGCAaggAGCGUCUGG- -5' |
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| 27701 | 3' | -57.3 | NC_005882.1 | + | 21738 | 0.76 | 0.105119 |
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Target: 5'- gGUCCGCGCgcGCCGUcgccaguUCgaCGCGGACCu -3' miRNA: 3'- -UAGGCGUGa-CGGCA-------AGgaGCGUCUGG- -5' |
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| 27701 | 3' | -57.3 | NC_005882.1 | + | 32415 | 1.1 | 0.00028 |
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Target: 5'- cAUCCGCACUGCCGUUCCUCGCAGACCa -3' miRNA: 3'- -UAGGCGUGACGGCAAGGAGCGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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