Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27702 | 3' | -54.5 | NC_005882.1 | + | 1663 | 0.67 | 0.59246 |
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Target: 5'- uUCGCCGGUGGCgGGa-UUCCguccGGu -3' miRNA: 3'- -GGUGGCCACCGaCCgcAAGGauuaCC- -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 4315 | 0.67 | 0.591339 |
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Target: 5'- uUACgGGUGGCUGGUGcgagaaCCUGuuugcgcaguaacAUGGa -3' miRNA: 3'- gGUGgCCACCGACCGCaa----GGAU-------------UACC- -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 21153 | 0.67 | 0.581273 |
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Target: 5'- gCGCCGGUcaaccuGCUcGGCGUcaUCCUGgucAUGGc -3' miRNA: 3'- gGUGGCCAc-----CGA-CCGCA--AGGAU---UACC- -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 33475 | 0.68 | 0.559041 |
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Target: 5'- gCCACCGGUGGCagcguaagGGUGaUCCc----- -3' miRNA: 3'- -GGUGGCCACCGa-------CCGCaAGGauuacc -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 12948 | 0.71 | 0.367149 |
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Target: 5'- gCGCCGGcGGC-GGCGUUCCa----- -3' miRNA: 3'- gGUGGCCaCCGaCCGCAAGGauuacc -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 13075 | 0.74 | 0.237993 |
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Target: 5'- gCugCGGcGGCUGGCGUcgaCgUGGUGGg -3' miRNA: 3'- gGugGCCaCCGACCGCAa--GgAUUACC- -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 5585 | 0.76 | 0.196954 |
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Target: 5'- aCCuguCCGGUGGCacgGGCGUUgccaaCCUuGUGGa -3' miRNA: 3'- -GGu--GGCCACCGa--CCGCAA-----GGAuUACC- -5' |
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| 27702 | 3' | -54.5 | NC_005882.1 | + | 33428 | 1.12 | 0.000509 |
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Target: 5'- gCCACCGGUGGCUGGCGUUCCUAAUGGu -3' miRNA: 3'- -GGUGGCCACCGACCGCAAGGAUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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