Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27702 | 5' | -56.4 | NC_005882.1 | + | 9276 | 0.66 | 0.565364 |
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Target: 5'- gGCAuUCGGGGCGCUGG-CGgagGUGAc -3' miRNA: 3'- gUGUuAGCCUCGCGGCCaGUgg-CACU- -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 12604 | 0.66 | 0.565364 |
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Target: 5'- -cCGAUCGccgcAGCGCCGGcCGCCGc-- -3' miRNA: 3'- guGUUAGCc---UCGCGGCCaGUGGCacu -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 15279 | 0.68 | 0.449075 |
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Target: 5'- gUACGAgCGG-GCGucgaCCGGgagCGCCGUGAg -3' miRNA: 3'- -GUGUUaGCCuCGC----GGCCa--GUGGCACU- -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 18207 | 0.68 | 0.419653 |
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Target: 5'- gCGCAggCGcuGGUGCUGGUCACCGagGAc -3' miRNA: 3'- -GUGUuaGCc-UCGCGGCCAGUGGCa-CU- -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 27545 | 0.7 | 0.31373 |
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Target: 5'- cCGCAAUCGGcaucgGGCGCucgucggCGGUCGCCGc-- -3' miRNA: 3'- -GUGUUAGCC-----UCGCG-------GCCAGUGGCacu -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 21498 | 0.7 | 0.304401 |
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Target: 5'- gACGauGUCGGuauugaucgucgucGGCGCCGGUgCGCCGUa- -3' miRNA: 3'- gUGU--UAGCC--------------UCGCGGCCA-GUGGCAcu -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 3572 | 0.78 | 0.096978 |
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Target: 5'- gCACGAUCGcGucgcGUGCUGGUCAUCGUGAg -3' miRNA: 3'- -GUGUUAGC-Cu---CGCGGCCAGUGGCACU- -5' |
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| 27702 | 5' | -56.4 | NC_005882.1 | + | 33463 | 1.08 | 0.000595 |
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Target: 5'- uCACAAUCGGAGCGCCGGUCACCGUGAc -3' miRNA: 3'- -GUGUUAGCCUCGCGGCCAGUGGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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