Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 27705 | 3' | -57 | NC_005882.1 | + | 21996 | 0.67 | 0.484966 |
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Target: 5'- cCGUCGCGAUCGUgcugcGCCagcUCaacgGCCGcGg -3' miRNA: 3'- cGCAGCGCUAGCGa----CGGa--AGa---CGGC-C- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 7529 | 0.67 | 0.484966 |
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Target: 5'- uGCGUacucuuugGCGGUUGCUGCgaCUUCgUGCuCGGc -3' miRNA: 3'- -CGCAg-------CGCUAGCGACG--GAAG-ACG-GCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 13471 | 0.67 | 0.46458 |
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Target: 5'- uGCGcCGCGaAUCGCUGCgCUgguaUCUGauuCUGGc -3' miRNA: 3'- -CGCaGCGC-UAGCGACG-GA----AGAC---GGCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 18347 | 0.67 | 0.44465 |
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Target: 5'- cGCGUCGCG-UCGCaagGCCggUCUGauGa -3' miRNA: 3'- -CGCAGCGCuAGCGa--CGGa-AGACggCc -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 27121 | 0.66 | 0.548445 |
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Target: 5'- cGCGUCcuggGCGGccagcUCGUgaacgGCCUgaugCUGgCCGGg -3' miRNA: 3'- -CGCAG----CGCU-----AGCGa----CGGAa---GAC-GGCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 15414 | 0.66 | 0.505769 |
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Target: 5'- ----gGCGAUCuGCU-CCUgCUGCCGGa -3' miRNA: 3'- cgcagCGCUAG-CGAcGGAaGACGGCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 27661 | 0.66 | 0.516314 |
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Target: 5'- aCGcCGCGGcCGCgGCCUgcgucgccaUGCCGGc -3' miRNA: 3'- cGCaGCGCUaGCGaCGGAag-------ACGGCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 3623 | 0.66 | 0.544122 |
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Target: 5'- cGCGaCGCGAUCG-UGCCgaaacggugucgCUGCUGu -3' miRNA: 3'- -CGCaGCGCUAGCgACGGaa----------GACGGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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