Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27705 | 3' | -57 | NC_005882.1 | + | 7386 | 0.68 | 0.39702 |
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Target: 5'- uCGUCGCGA-CGUUGUUggugCUGCuCGGc -3' miRNA: 3'- cGCAGCGCUaGCGACGGaa--GACG-GCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 3869 | 0.68 | 0.406283 |
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Target: 5'- aGCGUCGCGAUCGgCagcgcgUGCgCUUC-GUCGa -3' miRNA: 3'- -CGCAGCGCUAGC-G------ACG-GAAGaCGGCc -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 8052 | 0.68 | 0.406283 |
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Target: 5'- uCGUCGCGAUCGUUucGUCUgcauugGCCGa -3' miRNA: 3'- cGCAGCGCUAGCGA--CGGAaga---CGGCc -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 8854 | 0.68 | 0.425209 |
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Target: 5'- uGCGaCGCGGUgucgaggcaaUGCgaGCgCUUUUGCCGGg -3' miRNA: 3'- -CGCaGCGCUA----------GCGa-CG-GAAGACGGCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 18347 | 0.67 | 0.44465 |
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Target: 5'- cGCGUCGCG-UCGCaagGCCggUCUGauGa -3' miRNA: 3'- -CGCAGCGCuAGCGa--CGGa-AGACggCc -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 13471 | 0.67 | 0.46458 |
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Target: 5'- uGCGcCGCGaAUCGCUGCgCUgguaUCUGauuCUGGc -3' miRNA: 3'- -CGCaGCGC-UAGCGACG-GA----AGAC---GGCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 7529 | 0.67 | 0.484966 |
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Target: 5'- uGCGUacucuuugGCGGUUGCUGCgaCUUCgUGCuCGGc -3' miRNA: 3'- -CGCAg-------CGCUAGCGACG--GAAG-ACG-GCC- -5' |
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| 27705 | 3' | -57 | NC_005882.1 | + | 35836 | 1.13 | 0.000247 |
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Target: 5'- aGCGUCGCGAUCGCUGCCUUCUGCCGGa -3' miRNA: 3'- -CGCAGCGCUAGCGACGGAAGACGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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