Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2771 | 3' | -59.3 | NC_001491.2 | + | 114579 | 0.66 | 0.772623 |
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Target: 5'- cCCGUguaccucgCCUGCGGccGGCgcggcgCugGGGCCg -3' miRNA: 3'- -GGCGa-------GGACGUCu-CCGa-----GugCCUGGg -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 148867 | 0.66 | 0.790566 |
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Target: 5'- -gGCUCCgGCAGGcacaGCUC-CGuGGCCCc -3' miRNA: 3'- ggCGAGGaCGUCUc---CGAGuGC-CUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 42534 | 0.66 | 0.790566 |
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Target: 5'- gCCGCagCCUGgGcGGGCUCuguaGGGCuCCa -3' miRNA: 3'- -GGCGa-GGACgUcUCCGAGug--CCUG-GG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 114721 | 0.66 | 0.79934 |
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Target: 5'- gCGCgUCCUGCccgacuacGAcGGCUgcaaggacaUGCGGGCCCa -3' miRNA: 3'- gGCG-AGGACGu-------CU-CCGA---------GUGCCUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 145118 | 0.66 | 0.79934 |
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Target: 5'- aUGCUCCggaccCGGGGuGC-CGgGGACCCu -3' miRNA: 3'- gGCGAGGac---GUCUC-CGaGUgCCUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 144447 | 0.66 | 0.801945 |
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Target: 5'- gCCGCUUCUcuacccuggaaaaGCAGAGGCggaaaagauguugagUUggaGCGGAgCCg -3' miRNA: 3'- -GGCGAGGA-------------CGUCUCCG---------------AG---UGCCUgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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