Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2771 | 3' | -59.3 | NC_001491.2 | + | 75199 | 0.69 | 0.597842 |
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Target: 5'- -aGCUgUUcCAGAGGCUCGCGauccccucccaGACCCa -3' miRNA: 3'- ggCGAgGAcGUCUCCGAGUGC-----------CUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 82931 | 0.69 | 0.587961 |
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Target: 5'- gCGCcgUCCUGCGGuuGGggCACGGGCUg -3' miRNA: 3'- gGCG--AGGACGUCu-CCgaGUGCCUGGg -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 122576 | 0.7 | 0.578111 |
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Target: 5'- aCCGCUCCcccaGCcGGGGCUCGgaGcACCCa -3' miRNA: 3'- -GGCGAGGa---CGuCUCCGAGUg-CcUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 114373 | 0.72 | 0.464268 |
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Target: 5'- cUCGgUCCUGCacauGGGGGC-CACGGAgCUg -3' miRNA: 3'- -GGCgAGGACG----UCUCCGaGUGCCUgGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 88673 | 0.73 | 0.411839 |
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Target: 5'- cCCGgcCUCC-GUGGAGGCUUAUguGGACCCc -3' miRNA: 3'- -GGC--GAGGaCGUCUCCGAGUG--CCUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 85874 | 0.73 | 0.386226 |
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Target: 5'- gCCGCcacacacgCCUGCaucacggGGGGGUUgAUGGACCCg -3' miRNA: 3'- -GGCGa-------GGACG-------UCUCCGAgUGCCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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