Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2771 | 3' | -59.3 | NC_001491.2 | + | 144447 | 0.66 | 0.801945 |
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Target: 5'- gCCGCUUCUcuacccuggaaaaGCAGAGGCggaaaagauguugagUUggaGCGGAgCCg -3' miRNA: 3'- -GGCGAGGA-------------CGUCUCCG---------------AG---UGCCUgGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 145118 | 0.66 | 0.79934 |
|
Target: 5'- aUGCUCCggaccCGGGGuGC-CGgGGACCCu -3' miRNA: 3'- gGCGAGGac---GUCUC-CGaGUgCCUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 146621 | 0.67 | 0.735385 |
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Target: 5'- aCGCcCCgccGCGGAGGCgCAggaGGGCCUc -3' miRNA: 3'- gGCGaGGa--CGUCUCCGaGUg--CCUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 146907 | 0.69 | 0.617667 |
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Target: 5'- aCGgUCCagGCcGAGGC-C-CGGGCCCg -3' miRNA: 3'- gGCgAGGa-CGuCUCCGaGuGCCUGGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 147354 | 0.68 | 0.64548 |
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Target: 5'- gCCGCUCUUGCcccuGGGGCgcccguggauggCACGGucgUCCg -3' miRNA: 3'- -GGCGAGGACGu---CUCCGa-----------GUGCCu--GGG- -5' |
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| 2771 | 3' | -59.3 | NC_001491.2 | + | 148867 | 0.66 | 0.790566 |
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Target: 5'- -gGCUCCgGCAGGcacaGCUC-CGuGGCCCc -3' miRNA: 3'- ggCGAGGaCGUCUc---CGAGuGC-CUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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