Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2771 | 3' | -59.3 | NC_001491.2 | + | 116345 | 0.66 | 0.771713 |
Target: 5'- gCCGCUggUGCgcuacaacgccgaGGGGGC-C-CGGGCCCg -3' miRNA: 3'- -GGCGAggACG-------------UCUCCGaGuGCCUGGG- -5' |
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2771 | 3' | -59.3 | NC_001491.2 | + | 47593 | 0.66 | 0.76347 |
Target: 5'- gCCGC-CCUGU-GAGGaUCGCGG-CCg -3' miRNA: 3'- -GGCGaGGACGuCUCCgAGUGCCuGGg -5' |
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2771 | 3' | -59.3 | NC_001491.2 | + | 146621 | 0.67 | 0.735385 |
Target: 5'- aCGCcCCgccGCGGAGGCgCAggaGGGCCUc -3' miRNA: 3'- gGCGaGGa--CGUCUCCGaGUg--CCUGGG- -5' |
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2771 | 3' | -59.3 | NC_001491.2 | + | 86768 | 0.67 | 0.735385 |
Target: 5'- aCCGCuUCCgggGUuaguuggcgguuGGGGGacgaCGCGGACCCc -3' miRNA: 3'- -GGCG-AGGa--CG------------UCUCCga--GUGCCUGGG- -5' |
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2771 | 3' | -59.3 | NC_001491.2 | + | 102986 | 0.67 | 0.735385 |
Target: 5'- cCCGCUUUUGCAGcuccucgauAGGag-GCGGGCUCg -3' miRNA: 3'- -GGCGAGGACGUC---------UCCgagUGCCUGGG- -5' |
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2771 | 3' | -59.3 | NC_001491.2 | + | 85874 | 0.73 | 0.386226 |
Target: 5'- gCCGCcacacacgCCUGCaucacggGGGGGUUgAUGGACCCg -3' miRNA: 3'- -GGCGa-------GGACG-------UCUCCGAgUGCCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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