Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2771 | 5' | -59.6 | NC_001491.2 | + | 60616 | 0.66 | 0.723407 |
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Target: 5'- uGAGGgCCUCucuGUuGCUGguguCUGGGUCc -3' miRNA: 3'- uCUCCgGGAGuu-CGuCGAC----GACCCAG- -5' |
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| 2771 | 5' | -59.6 | NC_001491.2 | + | 116733 | 0.66 | 0.713498 |
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Target: 5'- --cGGCCCUCGGGCcccgacgcaaGGCUGCcGcGGcCg -3' miRNA: 3'- ucuCCGGGAGUUCG----------UCGACGaC-CCaG- -5' |
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| 2771 | 5' | -59.6 | NC_001491.2 | + | 146419 | 0.67 | 0.632482 |
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Target: 5'- cGGGG-CCUCGGGCuGUUGCUGGc-- -3' miRNA: 3'- uCUCCgGGAGUUCGuCGACGACCcag -5' |
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| 2771 | 5' | -59.6 | NC_001491.2 | + | 147538 | 0.69 | 0.531519 |
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Target: 5'- cGGGGCCCUC-GGCuGGCa--UGGGUCc -3' miRNA: 3'- uCUCCGGGAGuUCG-UCGacgACCCAG- -5' |
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| 2771 | 5' | -59.6 | NC_001491.2 | + | 100422 | 0.69 | 0.502244 |
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Target: 5'- cGAGGCCCagucugaCGAGgAGCaGCUGGG-Ca -3' miRNA: 3'- uCUCCGGGa------GUUCgUCGaCGACCCaG- -5' |
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| 2771 | 5' | -59.6 | NC_001491.2 | + | 24053 | 0.7 | 0.473697 |
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Target: 5'- --uGGCCaCUCuaguGGCAGCcagUGuCUGGGUCg -3' miRNA: 3'- ucuCCGG-GAGu---UCGUCG---AC-GACCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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