miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27717 3' -43.6 NC_005884.1 + 273 0.66 0.998318
Target:  5'- gAGUCAag-GUugcccAAGGUUUUGGCCCa -3'
miRNA:   3'- aUCAGUguaUAuu---UUUUAGGACCGGG- -5'
27717 3' -43.6 NC_005884.1 + 3789 0.67 0.997653
Target:  5'- cUAGUUACcgGUGcaauccuggaagaugGAGAAUCCUuguGGCCg -3'
miRNA:   3'- -AUCAGUGuaUAU---------------UUUUUAGGA---CCGGg -5'
27717 3' -43.6 NC_005884.1 + 27922 0.69 0.989628
Target:  5'- aAGUUAUcgAUAAAGGuUUCaGGCCCa -3'
miRNA:   3'- aUCAGUGuaUAUUUUUuAGGaCCGGG- -5'
27717 3' -43.6 NC_005884.1 + 9790 1.14 0.006172
Target:  5'- cUAGUCACAUAUAAAAAAUCCUGGCCCa -3'
miRNA:   3'- -AUCAGUGUAUAUUUUUUAGGACCGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.