Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27717 | 3' | -43.6 | NC_005884.1 | + | 273 | 0.66 | 0.998318 |
Target: 5'- gAGUCAag-GUugcccAAGGUUUUGGCCCa -3' miRNA: 3'- aUCAGUguaUAuu---UUUUAGGACCGGG- -5' |
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27717 | 3' | -43.6 | NC_005884.1 | + | 3789 | 0.67 | 0.997653 |
Target: 5'- cUAGUUACcgGUGcaauccuggaagaugGAGAAUCCUuguGGCCg -3' miRNA: 3'- -AUCAGUGuaUAU---------------UUUUUAGGA---CCGGg -5' |
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27717 | 3' | -43.6 | NC_005884.1 | + | 27922 | 0.69 | 0.989628 |
Target: 5'- aAGUUAUcgAUAAAGGuUUCaGGCCCa -3' miRNA: 3'- aUCAGUGuaUAUUUUUuAGGaCCGGG- -5' |
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27717 | 3' | -43.6 | NC_005884.1 | + | 9790 | 1.14 | 0.006172 |
Target: 5'- cUAGUCACAUAUAAAAAAUCCUGGCCCa -3' miRNA: 3'- -AUCAGUGUAUAUUUUUUAGGACCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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