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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27718 | 5' | -51.8 | NC_005884.1 | + | 10720 | 0.68 | 0.683137 |
Target: 5'- cAGCAUauaaGGCUAGCGggGAUGAuuCUUCu- -3' miRNA: 3'- -UCGUG----UCGGUCGCuuCUGUU--GAAGua -5' |
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27718 | 5' | -51.8 | NC_005884.1 | + | 10759 | 1.07 | 0.001819 |
Target: 5'- aAGCACAGCCAGCGAAGACAACUUCAUa -3' miRNA: 3'- -UCGUGUCGGUCGCUUCUGUUGAAGUA- -5' |
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27718 | 5' | -51.8 | NC_005884.1 | + | 15714 | 0.67 | 0.702807 |
Target: 5'- cAGCAUaguucuauacgggaAGCCGGUcgGAAGACuucGCUUCGUa -3' miRNA: 3'- -UCGUG--------------UCGGUCG--CUUCUGu--UGAAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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