Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2772 | 3' | -53.2 | NC_001491.2 | + | 13183 | 0.67 | 0.955777 |
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Target: 5'- aGGCGaGGCgCGcUGUUUGGgAGCGCa- -3' miRNA: 3'- gUUGC-CCGgGC-ACAAACUgUUGCGac -5' |
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| 2772 | 3' | -53.2 | NC_001491.2 | + | 54824 | 0.67 | 0.955777 |
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Target: 5'- aAGCGGGUCUGUGgua-ACAcUGCUGg -3' miRNA: 3'- gUUGCCCGGGCACaaacUGUuGCGAC- -5' |
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| 2772 | 3' | -53.2 | NC_001491.2 | + | 116366 | 0.68 | 0.9279 |
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Target: 5'- gGugGuGGCCCGgaccgUGGCGcCGCUGg -3' miRNA: 3'- gUugC-CCGGGCacaa-ACUGUuGCGAC- -5' |
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| 2772 | 3' | -53.2 | NC_001491.2 | + | 112953 | 0.68 | 0.904361 |
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Target: 5'- -cGCGGGCCCGUGacccUUGACcagauAugGCc- -3' miRNA: 3'- guUGCCCGGGCACa---AACUG-----UugCGac -5' |
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| 2772 | 3' | -53.2 | NC_001491.2 | + | 82464 | 0.7 | 0.853952 |
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Target: 5'- aAACGGGCCUGUGUUcuguaUGA--ACGCc- -3' miRNA: 3'- gUUGCCCGGGCACAA-----ACUguUGCGac -5' |
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| 2772 | 3' | -53.2 | NC_001491.2 | + | 59383 | 0.7 | 0.829091 |
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Target: 5'- uGACGGGCCUGUGcgcuacccaUGGCAGCGg-- -3' miRNA: 3'- gUUGCCCGGGCACaa-------ACUGUUGCgac -5' |
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| 2772 | 3' | -53.2 | NC_001491.2 | + | 121415 | 0.71 | 0.81157 |
|
Target: 5'- -cACGGcuGUCCGUGg--GGCAugGCUGg -3' miRNA: 3'- guUGCC--CGGGCACaaaCUGUugCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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