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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27720 | 3' | -49.5 | NC_005884.1 | + | 16127 | 0.66 | 0.915588 |
Target: 5'- -gACGCUGUucggcgguuccaCCACUAGCUAGCUg- -3' miRNA: 3'- agUGUGAUAug----------GGUGAUCGGUUGAgu -5' |
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27720 | 3' | -49.5 | NC_005884.1 | + | 21140 | 0.66 | 0.909448 |
Target: 5'- uUCAC-CUGUccgGCCCAuaugcugaucuucuCUAGCCuuaaGACUCAu -3' miRNA: 3'- -AGUGuGAUA---UGGGU--------------GAUCGG----UUGAGU- -5' |
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27720 | 3' | -49.5 | NC_005884.1 | + | 30183 | 0.66 | 0.897162 |
Target: 5'- -aGCACUAUcaucaguguaACCCugaccCUGGCCAGCggUCAu -3' miRNA: 3'- agUGUGAUA----------UGGGu----GAUCGGUUG--AGU- -5' |
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27720 | 3' | -49.5 | NC_005884.1 | + | 41950 | 0.69 | 0.787544 |
Target: 5'- aUAUGCgacUACCC-CUAGCCAACUgGa -3' miRNA: 3'- aGUGUGau-AUGGGuGAUCGGUUGAgU- -5' |
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27720 | 3' | -49.5 | NC_005884.1 | + | 23684 | 1.1 | 0.002115 |
Target: 5'- gUCACACUAUACCCACUAGCCAACUCAg -3' miRNA: 3'- -AGUGUGAUAUGGGUGAUCGGUUGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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