miRNA: 3'- auUUAGAGGgaUAGACCGGUCUGaCC- -5'
miRNA: 3'- auUUAGAGGGA-UAGaCCG-GUCUGacc -5'
miRNA: 3'- -AUUUAGAGGGAUAGACCGGUCUGACC- -5'
miRNA: 3'- aUUUAGAGGGAUa-GACCGg------UCUGACC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27726 | 5' | -51 | NC_005884.1 | + | 42314 | 0.79 | 0.221471 |
|
Target: 5'- -cAAUCUCCa-GUCUGGCCAGAUaGGg -3' miRNA: 3'- auUUAGAGGgaUAGACCGGUCUGaCC- -5' |
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| 27726 | 5' | -51 | NC_005884.1 | + | 8198 | 0.69 | 0.704491 |
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Target: 5'- -uGAUCUCCCUgGUCcGGCuCAGGCa-- -3' miRNA: 3'- auUUAGAGGGA-UAGaCCG-GUCUGacc -5' |
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| 27726 | 5' | -51 | NC_005884.1 | + | 42389 | 1.1 | 0.001529 |
|
Target: 5'- gUAAAUCUCCCUAUCUGGCCAGACUGGa -3' miRNA: 3'- -AUUUAGAGGGAUAGACCGGUCUGACC- -5' |
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| 27726 | 5' | -51 | NC_005884.1 | + | 41574 | 0.75 | 0.339969 |
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Target: 5'- cAAGUUUCCCUAgaCUGGCaagggcaAGACUGGc -3' miRNA: 3'- aUUUAGAGGGAUa-GACCGg------UCUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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