Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27727 | 3' | -49.7 | NC_005884.1 | + | 12351 | 0.66 | 0.911948 |
Target: 5'- uUUCUACUCcaauGCuCUCAAgg-CGGCCa -3' miRNA: 3'- -AGGAUGAGauu-CG-GAGUUagaGUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 38914 | 0.66 | 0.902106 |
Target: 5'- cUCCUuggcGCUCUuacuauuccauCCUCAGUUggaUCAGCCg -3' miRNA: 3'- -AGGA----UGAGAuuc--------GGAGUUAG---AGUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 24803 | 0.67 | 0.890125 |
Target: 5'- uUCCUuaaCUGAGCUUCAAUCacUAGCUu -3' miRNA: 3'- -AGGAugaGAUUCGGAGUUAGa-GUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 28227 | 0.67 | 0.874083 |
Target: 5'- uUCUgaaUCUGAGUCcCAAUCcaggCAGCCg -3' miRNA: 3'- -AGGaugAGAUUCGGaGUUAGa---GUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 42618 | 0.68 | 0.838666 |
Target: 5'- gCCUuuCUCUuucaGAGCCaUCAAUUgagUAGCCa -3' miRNA: 3'- aGGAu-GAGA----UUCGG-AGUUAGa--GUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 21038 | 0.68 | 0.829171 |
Target: 5'- aUCCaGCUUggaaauAGCCUCAGaauauccuuuacUCUgAGCCa -3' miRNA: 3'- -AGGaUGAGau----UCGGAGUU------------AGAgUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 41622 | 0.68 | 0.819442 |
Target: 5'- uUCCUACUCaagcgcAGCUUUcuAAUUUCAGCa -3' miRNA: 3'- -AGGAUGAGau----UCGGAG--UUAGAGUCGg -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 18010 | 0.68 | 0.809489 |
Target: 5'- cUUCUAC-CUGAGCUUUgGAUC-CAGCCu -3' miRNA: 3'- -AGGAUGaGAUUCGGAG-UUAGaGUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 35897 | 0.71 | 0.665775 |
Target: 5'- ---aGCUCUAcAGUCUCAGUCUguuUAGCCu -3' miRNA: 3'- aggaUGAGAU-UCGGAGUUAGA---GUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 9061 | 0.72 | 0.595584 |
Target: 5'- uUCCUGCUCaguagcauggAAGUCcaugCAAUCUCGGCg -3' miRNA: 3'- -AGGAUGAGa---------UUCGGa---GUUAGAGUCGg -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 29231 | 0.73 | 0.560807 |
Target: 5'- aCCUGCcauugCUGAGUCUCAAUgUCcccGCCa -3' miRNA: 3'- aGGAUGa----GAUUCGGAGUUAgAGu--CGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 21392 | 0.75 | 0.439898 |
Target: 5'- cCCUuccCUCaGAGCCUUAuaCUCAGCCg -3' miRNA: 3'- aGGAu--GAGaUUCGGAGUuaGAGUCGG- -5' |
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27727 | 3' | -49.7 | NC_005884.1 | + | 42733 | 1.13 | 0.001501 |
Target: 5'- cUCCUACUCUAAGCCUCAAUCUCAGCCc -3' miRNA: 3'- -AGGAUGAGAUUCGGAGUUAGAGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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