miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27729 5' -52.8 NC_005884.1 + 2372 0.66 0.730847
Target:  5'- uGCua-CUUGGGCggccuuuaauccUGuCGCCAGCAGACg -3'
miRNA:   3'- gUGuaaGAGUCCG------------AC-GCGGUCGUUUG- -5'
27729 5' -52.8 NC_005884.1 + 517 1.09 0.001069
Target:  5'- gCACAUUCUCAGGCUGCGCCAGCAAACc -3'
miRNA:   3'- -GUGUAAGAGUCCGACGCGGUCGUUUG- -5'
27729 5' -52.8 NC_005884.1 + 612 0.68 0.597299
Target:  5'- --aAUUCgcucCAGGCUGCGauaagagcgcggcgcUCAGCAAACa -3'
miRNA:   3'- gugUAAGa---GUCCGACGC---------------GGUCGUUUG- -5'
27729 5' -52.8 NC_005884.1 + 18114 0.67 0.650737
Target:  5'- gAgAUUgUCcagAGGCaGCGCCAGCAGGa -3'
miRNA:   3'- gUgUAAgAG---UCCGaCGCGGUCGUUUg -5'
27729 5' -52.8 NC_005884.1 + 2547 0.67 0.6716
Target:  5'- cCGCAgu-UCAGGUccuauccaacagGCGCCAGCAAGa -3'
miRNA:   3'- -GUGUaagAGUCCGa-----------CGCGGUCGUUUg -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.