Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
2773 | 5' | -60.5 | NC_001491.2 | + | 70060 | 0.72 | 0.383452 |
Target: 5'- -gUUACUUGCCGCCUgaCCGgcgcCCCGCUGg -3' miRNA: 3'- ugAAUGAGCGGUGGGg-GGU----GGGCGAC- -5' |
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2773 | 5' | -60.5 | NC_001491.2 | + | 125164 | 0.72 | 0.373029 |
Target: 5'- cGCUcGCUCGCCGCCgcaaugccaacccgCCCCACCuCGUcuUGg -3' miRNA: 3'- -UGAaUGAGCGGUGG--------------GGGGUGG-GCG--AC- -5' |
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2773 | 5' | -60.5 | NC_001491.2 | + | 125547 | 0.75 | 0.228237 |
Target: 5'- gGCggcGCUCGCgCGCUCCCgCGCCCGCUc -3' miRNA: 3'- -UGaa-UGAGCG-GUGGGGG-GUGGGCGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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