miRNA: 3'- gAUUGACUaACGGaua--UGaACGAAUG- -5'
miRNA: 3'- gaUUGACUAACGGAUAUGAaCgAAUG- -5'
miRNA: 3'- -GAUUGACUAACGGAUAUGAACGAAUG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27730 | 5' | -42.9 | NC_005884.1 | + | 18101 | 0.68 | 0.996997 |
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Target: 5'- aUAACUGAcUGCCcaaaaGC-UGCUUGCc -3' miRNA: 3'- gAUUGACUaACGGaua--UGaACGAAUG- -5' |
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| 27730 | 5' | -42.9 | NC_005884.1 | + | 1160 | 0.79 | 0.655857 |
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Target: 5'- -aAAUUGGUUGCCUAUGCUgGgUUACu -3' miRNA: 3'- gaUUGACUAACGGAUAUGAaCgAAUG- -5' |
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| 27730 | 5' | -42.9 | NC_005884.1 | + | 1127 | 1.11 | 0.010564 |
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Target: 5'- uCUAACUGAUUGCCUAUACUUGCUUACc -3' miRNA: 3'- -GAUUGACUAACGGAUAUGAACGAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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