miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27731 3' -39.7 NC_005884.1 + 1368 0.66 0.999997
Target:  5'- -gUCCUGACUGGuccuaAUCAGGGUa----- -3'
miRNA:   3'- ugAGGAUUGAUC-----UAGUUUCAauuaau -5'
27731 3' -39.7 NC_005884.1 + 1897 1.09 0.032993
Target:  5'- aACUCCUAACUAGAUCAAAGUUAAUUAa -3'
miRNA:   3'- -UGAGGAUUGAUCUAGUUUCAAUUAAU- -5'
27731 3' -39.7 NC_005884.1 + 2003 0.75 0.974519
Target:  5'- gACUUCUAACUAGGUCAGacuagaaugaccGGUgUAGUUAa -3'
miRNA:   3'- -UGAGGAUUGAUCUAGUU------------UCA-AUUAAU- -5'
27731 3' -39.7 NC_005884.1 + 13112 0.66 0.999999
Target:  5'- cACUCCU--CUAGAUCcAGGUcAAUUc -3'
miRNA:   3'- -UGAGGAuuGAUCUAGuUUCAaUUAAu -5'
27731 3' -39.7 NC_005884.1 + 34463 0.69 0.999842
Target:  5'- aACUCCUAACUacaaauacucAGAUCAGgugauaGGUUAu--- -3'
miRNA:   3'- -UGAGGAUUGA----------UCUAGUU------UCAAUuaau -5'
27731 3' -39.7 NC_005884.1 + 39868 0.73 0.992148
Target:  5'- -aUCCc--CUAGAUCAAAGUUAGUa- -3'
miRNA:   3'- ugAGGauuGAUCUAGUUUCAAUUAau -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.