Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27733 | 3' | -46.1 | NC_005884.1 | + | 35803 | 0.66 | 0.989795 |
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Target: 5'- --aCUCAgaugauggaAGGACUgGACcUGAAGUCUg -3' miRNA: 3'- uagGAGU---------UCUUGAgUUGuACUUCAGG- -5' |
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| 27733 | 3' | -46.1 | NC_005884.1 | + | 14141 | 0.66 | 0.989327 |
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Target: 5'- gAUCCUCAAauccauGGACUCAAUcaggauacagcuuuGUGGcgauaugaagcuGGUCCa -3' miRNA: 3'- -UAGGAGUU------CUUGAGUUG--------------UACU------------UCAGG- -5' |
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| 27733 | 3' | -46.1 | NC_005884.1 | + | 30693 | 0.67 | 0.982081 |
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Target: 5'- uUCUUCGAGGaggucauccagAUUCAugAUGGAGaCCa -3' miRNA: 3'- uAGGAGUUCU-----------UGAGUugUACUUCaGG- -5' |
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| 27733 | 3' | -46.1 | NC_005884.1 | + | 19016 | 0.68 | 0.954718 |
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Target: 5'- cUCCcaUCAGGcuaAGCUCAACAgccacuGAGUCCa -3' miRNA: 3'- uAGG--AGUUC---UUGAGUUGUac----UUCAGG- -5' |
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| 27733 | 3' | -46.1 | NC_005884.1 | + | 8558 | 0.68 | 0.954718 |
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Target: 5'- -aCCaUUAGGAACUCGACGaaGAauAGUCCc -3' miRNA: 3'- uaGG-AGUUCUUGAGUUGUa-CU--UCAGG- -5' |
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| 27733 | 3' | -46.1 | NC_005884.1 | + | 19963 | 0.69 | 0.944901 |
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Target: 5'- uGUUCUCuggugauAGggUUCAACugguaGAAGUCCa -3' miRNA: 3'- -UAGGAGu------UCuuGAGUUGua---CUUCAGG- -5' |
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| 27733 | 3' | -46.1 | NC_005884.1 | + | 8453 | 1.12 | 0.004415 |
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Target: 5'- aAUCCUCAAGAACUCAACAUGAAGUCCu -3' miRNA: 3'- -UAGGAGUUCUUGAGUUGUACUUCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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