miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27736 3' -42.9 NC_005884.1 + 31564 1.11 0.012296
Target:  5'- aUAAGAGUUCCGAAAUACUUACCAAGGu -3'
miRNA:   3'- -AUUCUCAAGGCUUUAUGAAUGGUUCC- -5'
27736 3' -42.9 NC_005884.1 + 14649 0.69 0.995327
Target:  5'- cAGGuGUUCCGccuGAUACUgcgGCCAucgguaaaAGGg -3'
miRNA:   3'- aUUCuCAAGGCu--UUAUGAa--UGGU--------UCC- -5'
27736 3' -42.9 NC_005884.1 + 14985 0.67 0.999269
Target:  5'- aGGGAGUUCUGAuuuuggauucccaGAUACUUucCCAAcuuGGg -3'
miRNA:   3'- aUUCUCAAGGCU-------------UUAUGAAu-GGUU---CC- -5'
27736 3' -42.9 NC_005884.1 + 5738 0.66 0.999442
Target:  5'- ------cUCUGGAAUGCUUAuauCCAGGGa -3'
miRNA:   3'- auucucaAGGCUUUAUGAAU---GGUUCC- -5'
27736 3' -42.9 NC_005884.1 + 6396 0.67 0.999095
Target:  5'- --uGAGUUCCGAAugccugcagaGUAgUcuCCGAGGa -3'
miRNA:   3'- auuCUCAAGGCUU----------UAUgAauGGUUCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.