miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27736 5' -51.8 NC_005884.1 + 2637 0.66 0.79798
Target:  5'- uAGCCUUAG-CCUcuugcugGCGCcuGUUGGAUAGGa -3'
miRNA:   3'- -UCGGAAUCaGGG-------CGUG--UAGCCUAUUU- -5'
27736 5' -51.8 NC_005884.1 + 26914 0.67 0.755583
Target:  5'- aAGCCaacuauuaauuuaUUAGgagCCUGCACAcCGGAUAc- -3'
miRNA:   3'- -UCGG-------------AAUCa--GGGCGUGUaGCCUAUuu -5'
27736 5' -51.8 NC_005884.1 + 31601 1.06 0.002123
Target:  5'- aAGCCUUAGUCCCGCACAUCGGAUAAAu -3'
miRNA:   3'- -UCGGAAUCAGGGCGUGUAGCCUAUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.