miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27737 5' -52.1 NC_005884.1 + 36649 1.06 0.002071
Target:  5'- uAAAGUCUGCUCAGACUUCGCCAACCGu -3'
miRNA:   3'- -UUUCAGACGAGUCUGAAGCGGUUGGC- -5'
27737 5' -52.1 NC_005884.1 + 5428 0.71 0.464944
Target:  5'- cGGGUUUGagaCUgAGACUUCGUCAACCa -3'
miRNA:   3'- uUUCAGAC---GAgUCUGAAGCGGUUGGc -5'
27737 5' -52.1 NC_005884.1 + 23943 0.68 0.645102
Target:  5'- gAAGGUaaggaGCUCAG-CUUCGCCAgACCu -3'
miRNA:   3'- -UUUCAga---CGAGUCuGAAGCGGU-UGGc -5'
27737 5' -52.1 NC_005884.1 + 21764 0.66 0.757225
Target:  5'- -cAGUCUGUUauuaaCAGGCUUUcugguagggauaaGCCAGCCa -3'
miRNA:   3'- uuUCAGACGA-----GUCUGAAG-------------CGGUUGGc -5'
27737 5' -52.1 NC_005884.1 + 17549 0.66 0.800324
Target:  5'- cAGGGUCUcGaCUCAuuACUUCGCUGACCu -3'
miRNA:   3'- -UUUCAGA-C-GAGUc-UGAAGCGGUUGGc -5'
27737 5' -52.1 NC_005884.1 + 18863 0.66 0.810371
Target:  5'- ----aCUGCUCAGACUggGaCAGCUGa -3'
miRNA:   3'- uuucaGACGAGUCUGAagCgGUUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.