Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2777 | 3' | -55.2 | NC_001491.2 | + | 61579 | 0.66 | 0.922198 |
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Target: 5'- aCCGUcuccGCGCCAauuGCCCcaUGUGgagcCGCCGAc -3' miRNA: 3'- -GGCGu---UGUGGU---UGGG--ACACa---GCGGUU- -5' |
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| 2777 | 3' | -55.2 | NC_001491.2 | + | 140975 | 0.67 | 0.897866 |
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Target: 5'- gCCGCaAGCGCCAACCCcccaaggGUGagCGUCc- -3' miRNA: 3'- -GGCG-UUGUGGUUGGGa------CACa-GCGGuu -5' |
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| 2777 | 3' | -55.2 | NC_001491.2 | + | 125152 | 0.67 | 0.862171 |
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Target: 5'- gCCGCAAUGCCAACCCgccccaccUCGUCu- -3' miRNA: 3'- -GGCGUUGUGGUUGGGacac----AGCGGuu -5' |
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| 2777 | 3' | -55.2 | NC_001491.2 | + | 61496 | 0.68 | 0.83816 |
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Target: 5'- gCCGCu-CACgCAGCgCUGUG-CGCCGc -3' miRNA: 3'- -GGCGuuGUG-GUUGgGACACaGCGGUu -5' |
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| 2777 | 3' | -55.2 | NC_001491.2 | + | 118199 | 0.68 | 0.829762 |
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Target: 5'- aCGCG--GCCGACCCg--GUCGCCc- -3' miRNA: 3'- gGCGUugUGGUUGGGacaCAGCGGuu -5' |
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| 2777 | 3' | -55.2 | NC_001491.2 | + | 21665 | 0.69 | 0.803497 |
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Target: 5'- gCCGCGGCACUGGCgCUG-GUCGUUc- -3' miRNA: 3'- -GGCGUUGUGGUUGgGACaCAGCGGuu -5' |
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| 2777 | 3' | -55.2 | NC_001491.2 | + | 92513 | 0.77 | 0.375688 |
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Target: 5'- aCCGaCAGCGCCAACucuCCUGUGUC-CCAGc -3' miRNA: 3'- -GGC-GUUGUGGUUG---GGACACAGcGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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