Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2784 | 3' | -55.1 | NC_001491.2 | + | 145601 | 0.66 | 0.939148 |
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Target: 5'- aGCCGAUUucggacgcUGGUccuUGGGGAgccggugaGGGCu -3' miRNA: 3'- gCGGCUGA--------ACCAac-ACCCCUaa------CCCG- -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 147159 | 0.66 | 0.934277 |
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Target: 5'- cCGCCG-CUcUGGgagUGcUGGGGGgacUGGGa -3' miRNA: 3'- -GCGGCuGA-ACCa--AC-ACCCCUa--ACCCg -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 144903 | 0.66 | 0.929167 |
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Target: 5'- aGCCgGGCUgGGUgccGGGGAcgccggcUGGGCg -3' miRNA: 3'- gCGG-CUGAaCCAacaCCCCUa------ACCCG- -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 12413 | 0.66 | 0.918227 |
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Target: 5'- aGCCGuGCUUGGg-GUGGuaAUUGGGa -3' miRNA: 3'- gCGGC-UGAACCaaCACCccUAACCCg -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 95819 | 0.68 | 0.878304 |
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Target: 5'- uGgCGGCUcaccacgUGGUUGaguaacaUGGGGGUaaagaUGGGCu -3' miRNA: 3'- gCgGCUGA-------ACCAAC-------ACCCCUA-----ACCCG- -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 41375 | 0.68 | 0.865084 |
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Target: 5'- aGCgCGGCUgccacacuUUGUGGGGGUgGGGUg -3' miRNA: 3'- gCG-GCUGAacc-----AACACCCCUAaCCCG- -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 121536 | 0.68 | 0.857442 |
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Target: 5'- gCGCUGGC-UGGagcGUGGGGAc-GGGUc -3' miRNA: 3'- -GCGGCUGaACCaa-CACCCCUaaCCCG- -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 112829 | 0.69 | 0.8163 |
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Target: 5'- gGCCGGagaUGGUUGgggagGGGGAaaaUGGGg -3' miRNA: 3'- gCGGCUga-ACCAACa----CCCCUa--ACCCg -5' |
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| 2784 | 3' | -55.1 | NC_001491.2 | + | 86930 | 0.74 | 0.539777 |
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Target: 5'- aCGgCGcACUUGGcggUGUGGGuGAgcUUGGGCg -3' miRNA: 3'- -GCgGC-UGAACCa--ACACCC-CU--AACCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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