Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2786 | 5' | -53.1 | NC_001491.2 | + | 68761 | 0.66 | 0.963032 |
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Target: 5'- aUGCCCuGGCgGCCCUcgaagauGCCGcGCG-UGCAg -3' miRNA: 3'- -ACGGG-UCG-UGGGA-------UGGUuUGUaACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 118312 | 0.66 | 0.959761 |
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Target: 5'- -cCCCGGCACCCaGCCcGGCuccggGCu -3' miRNA: 3'- acGGGUCGUGGGaUGGuUUGuaa--CGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 59495 | 0.66 | 0.959386 |
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Target: 5'- cGUUUAGUuuuagcgACCCUACCAGAgGUaGCAg -3' miRNA: 3'- aCGGGUCG-------UGGGAUGGUUUgUAaCGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 112949 | 0.66 | 0.955899 |
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Target: 5'- gGCCCGugACCCUugACCAGAUAUgGCc -3' miRNA: 3'- aCGGGUcgUGGGA--UGGUUUGUAaCGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 105502 | 0.66 | 0.955899 |
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Target: 5'- -uCCCAGCuuauaguaaggACCCcGCCGGACAUgcuacGCAa -3' miRNA: 3'- acGGGUCG-----------UGGGaUGGUUUGUAa----CGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 110865 | 0.66 | 0.951793 |
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Target: 5'- gGCCCAugcggccuagauGCAgCCUGCgGGAguUUGCGc -3' miRNA: 3'- aCGGGU------------CGUgGGAUGgUUUguAACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 51536 | 0.66 | 0.947438 |
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Target: 5'- --aCCAGCACgaaagcagUCUGCCAGACGUgUGCu -3' miRNA: 3'- acgGGUCGUG--------GGAUGGUUUGUA-ACGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 83268 | 0.66 | 0.963383 |
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Target: 5'- uUGUCCAGCcUCCcGCCAAccaGCGaUGCGu -3' miRNA: 3'- -ACGGGUCGuGGGaUGGUU---UGUaACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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