Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2786 | 5' | -53.1 | NC_001491.2 | + | 141582 | 0.69 | 0.852511 |
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Target: 5'- gGCCCuGCGCCCguaccuggcccggggGCCAGggGCGUucUGCGc -3' miRNA: 3'- aCGGGuCGUGGGa--------------UGGUU--UGUA--ACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 28903 | 0.69 | 0.851692 |
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Target: 5'- aUGCCCcgauaucgAGCACCUgcgcucGCUcAGCGUUGCAa -3' miRNA: 3'- -ACGGG--------UCGUGGGa-----UGGuUUGUAACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 59416 | 0.69 | 0.851692 |
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Target: 5'- gGCCU-GCACCg-ACCAAAgAUUGCGa -3' miRNA: 3'- aCGGGuCGUGGgaUGGUUUgUAACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 141809 | 0.7 | 0.808215 |
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Target: 5'- gGCCCAGCcauGCCCca-CGGACAgccgUGCGg -3' miRNA: 3'- aCGGGUCG---UGGGaugGUUUGUa---ACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 85347 | 0.74 | 0.625033 |
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Target: 5'- uUGCUgAGCACCCUaACCGGguuGCGUuuUGCAa -3' miRNA: 3'- -ACGGgUCGUGGGA-UGGUU---UGUA--ACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 81405 | 0.74 | 0.614394 |
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Target: 5'- gGCCCGGCGCuCCUuCCucuGGgGUUGCAu -3' miRNA: 3'- aCGGGUCGUG-GGAuGGu--UUgUAACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 85420 | 0.77 | 0.448209 |
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Target: 5'- gUGCCCcgcgGGCACCCUgucggaggccggacGCCAcGCAUUGCc -3' miRNA: 3'- -ACGGG----UCGUGGGA--------------UGGUuUGUAACGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 110587 | 0.78 | 0.387247 |
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Target: 5'- gUGCCCccGGUGCCCUAcCCAGACAUaGCGu -3' miRNA: 3'- -ACGGG--UCGUGGGAU-GGUUUGUAaCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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