Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2786 | 5' | -53.1 | NC_001491.2 | + | 112949 | 0.66 | 0.955899 |
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Target: 5'- gGCCCGugACCCUugACCAGAUAUgGCc -3' miRNA: 3'- aCGGGUcgUGGGA--UGGUUUGUAaCGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 105502 | 0.66 | 0.955899 |
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Target: 5'- -uCCCAGCuuauaguaaggACCCcGCCGGACAUgcuacGCAa -3' miRNA: 3'- acGGGUCG-----------UGGGaUGGUUUGUAa----CGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 110865 | 0.66 | 0.951793 |
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Target: 5'- gGCCCAugcggccuagauGCAgCCUGCgGGAguUUGCGc -3' miRNA: 3'- aCGGGU------------CGUgGGAUGgUUUguAACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 51536 | 0.66 | 0.947438 |
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Target: 5'- --aCCAGCACgaaagcagUCUGCCAGACGUgUGCu -3' miRNA: 3'- acgGGUCGUG--------GGAUGGUUUGUA-ACGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 65741 | 0.67 | 0.942831 |
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Target: 5'- aGCCgGGCGCCCguccACCAc-CAUcGCGg -3' miRNA: 3'- aCGGgUCGUGGGa---UGGUuuGUAaCGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 41381 | 0.67 | 0.937968 |
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Target: 5'- uUGUCCAGCGCggCUGCCAcACuUUGUg -3' miRNA: 3'- -ACGGGUCGUGg-GAUGGUuUGuAACGu -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 146893 | 0.67 | 0.932849 |
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Target: 5'- gGCCCGG-GCCCggGCCcccucGGCGUUGUAg -3' miRNA: 3'- aCGGGUCgUGGGa-UGGu----UUGUAACGU- -5' |
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| 2786 | 5' | -53.1 | NC_001491.2 | + | 110587 | 0.78 | 0.387247 |
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Target: 5'- gUGCCCccGGUGCCCUAcCCAGACAUaGCGu -3' miRNA: 3'- -ACGGG--UCGUGGGAU-GGUUUGUAaCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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