Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2787 | 3' | -48.9 | NC_001491.2 | + | 129998 | 0.66 | 0.998285 |
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Target: 5'- --uGAugUUgAcGUCGAUGUGGGAGUg -3' miRNA: 3'- cuuCUugAGgUuCAGCUGUACCCUUA- -5' |
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| 2787 | 3' | -48.9 | NC_001491.2 | + | 148803 | 0.66 | 0.997526 |
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Target: 5'- --cGGGCUCCcAGUCGACccgcGGGGc- -3' miRNA: 3'- cuuCUUGAGGuUCAGCUGua--CCCUua -5' |
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| 2787 | 3' | -48.9 | NC_001491.2 | + | 122952 | 0.66 | 0.997053 |
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Target: 5'- --cGGACUCCucgccGUCGACGgccaucgGGGAGc -3' miRNA: 3'- cuuCUUGAGGuu---CAGCUGUa------CCCUUa -5' |
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| 2787 | 3' | -48.9 | NC_001491.2 | + | 114420 | 0.67 | 0.995163 |
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Target: 5'- --cGAGCcCCGcgGGUCGAC-UGGGAGc -3' miRNA: 3'- cuuCUUGaGGU--UCAGCUGuACCCUUa -5' |
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| 2787 | 3' | -48.9 | NC_001491.2 | + | 99211 | 0.69 | 0.985107 |
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Target: 5'- gGAAGAGCgUCCAGGguacgGGCgAUGGGGAUu -3' miRNA: 3'- -CUUCUUG-AGGUUCag---CUG-UACCCUUA- -5' |
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| 2787 | 3' | -48.9 | NC_001491.2 | + | 129332 | 0.7 | 0.963827 |
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Target: 5'- gGAGGAugUgUggGUUGAUGUGGGGGg -3' miRNA: 3'- -CUUCUugAgGuuCAGCUGUACCCUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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