Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2788 | 5' | -53.8 | NC_001491.2 | + | 143703 | 0.67 | 0.920795 |
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Target: 5'- aAGCGCgucGCGCCAAcGGGAUgCCGAAu -3' miRNA: 3'- -UCGUGgu-UGCGGUUcCCUUAgGGUUU- -5' |
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| 2788 | 5' | -53.8 | NC_001491.2 | + | 105416 | 0.67 | 0.920795 |
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Target: 5'- cAGUACCGACccguccucggugGUCAGGGGGAcCCUAGu -3' miRNA: 3'- -UCGUGGUUG------------CGGUUCCCUUaGGGUUu -5' |
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| 2788 | 5' | -53.8 | NC_001491.2 | + | 26575 | 0.66 | 0.936508 |
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Target: 5'- aAGCACCGugcgguaaccgcuGCGCCAuccaGGGGAAaauugcgcUCCUGGu -3' miRNA: 3'- -UCGUGGU-------------UGCGGU----UCCCUU--------AGGGUUu -5' |
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| 2788 | 5' | -53.8 | NC_001491.2 | + | 145410 | 0.66 | 0.937012 |
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Target: 5'- aAGCuggaGCCGgagcggguGCGCCGGGGGAAgcggCgCCGGAg -3' miRNA: 3'- -UCG----UGGU--------UGCGGUUCCCUUa---G-GGUUU- -5' |
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| 2788 | 5' | -53.8 | NC_001491.2 | + | 147922 | 0.66 | 0.946545 |
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Target: 5'- cGCGCCGAgagcCGCCGGggcgggaggccGGGAGUCCg--- -3' miRNA: 3'- uCGUGGUU----GCGGUU-----------CCCUUAGGguuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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