Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2788 | 5' | -53.8 | NC_001491.2 | + | 147167 | 0.67 | 0.902276 |
Target: 5'- cGGCGCCucCGCCGcucuGGGAGUgCUggGg -3' miRNA: 3'- -UCGUGGuuGCGGUu---CCCUUAgGGuuU- -5' |
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2788 | 5' | -53.8 | NC_001491.2 | + | 64020 | 0.68 | 0.866466 |
Target: 5'- cAGCGCCGgcgGCGCCGAGaacGAGcguUCCCAc- -3' miRNA: 3'- -UCGUGGU---UGCGGUUCc--CUU---AGGGUuu -5' |
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2788 | 5' | -53.8 | NC_001491.2 | + | 65487 | 0.68 | 0.858602 |
Target: 5'- uGGCGCCAcaggUGCCAgccGGGGAAgcCCCGGg -3' miRNA: 3'- -UCGUGGUu---GCGGU---UCCCUUa-GGGUUu -5' |
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2788 | 5' | -53.8 | NC_001491.2 | + | 83798 | 0.69 | 0.842222 |
Target: 5'- gAGCACUuuuuccugAGCGCCcuuacGGGGAGUUCCGc- -3' miRNA: 3'- -UCGUGG--------UUGCGGu----UCCCUUAGGGUuu -5' |
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2788 | 5' | -53.8 | NC_001491.2 | + | 138941 | 0.79 | 0.300216 |
Target: 5'- gGGCGCCAGCGUaCAGGGGAGUguaCCCAc- -3' miRNA: 3'- -UCGUGGUUGCG-GUUCCCUUA---GGGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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