Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2790 | 3' | -54.3 | NC_001491.2 | + | 121545 | 0.71 | 0.765918 |
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Target: 5'- ---gGCGUCGAUGCGCUGGcuggaGCGUg -3' miRNA: 3'- aggaUGCGGCUGCGCGACCaaa--UGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 15789 | 0.72 | 0.736919 |
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Target: 5'- uUCCU-CGCCGGCGgGC-GGUgcaaaguccGCGCg -3' miRNA: 3'- -AGGAuGCGGCUGCgCGaCCAaa-------UGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 114567 | 0.72 | 0.686872 |
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Target: 5'- gCCUGCgGCCGGCgcgGCGCUGGggc-CGUg -3' miRNA: 3'- aGGAUG-CGGCUG---CGCGACCaaauGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 40355 | 0.77 | 0.438611 |
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Target: 5'- cUUCUAuuuCGCCGACGCGCUG--UUugGCa -3' miRNA: 3'- -AGGAU---GCGGCUGCGCGACcaAAugCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 114948 | 0.78 | 0.377961 |
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Target: 5'- gCCUGCGCCG-UGCGCUGGccccgggaGCGCc -3' miRNA: 3'- aGGAUGCGGCuGCGCGACCaaa-----UGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 141436 | 0.79 | 0.338228 |
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Target: 5'- cCCUGCGCagGGCGCuGCUGGauuUUUACGCc -3' miRNA: 3'- aGGAUGCGg-CUGCG-CGACC---AAAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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