Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2790 | 3' | -54.3 | NC_001491.2 | + | 121545 | 0.71 | 0.765918 |
Target: 5'- ---gGCGUCGAUGCGCUGGcuggaGCGUg -3' miRNA: 3'- aggaUGCGGCUGCGCGACCaaa--UGCG- -5' |
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2790 | 3' | -54.3 | NC_001491.2 | + | 123524 | 0.68 | 0.896782 |
Target: 5'- gCUUGCgGCCGcggGCGCGCaGGU--ACGCc -3' miRNA: 3'- aGGAUG-CGGC---UGCGCGaCCAaaUGCG- -5' |
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2790 | 3' | -54.3 | NC_001491.2 | + | 133382 | 0.68 | 0.909415 |
Target: 5'- aUCUGCgGuuGGCGCG-UGGUcaaguuUUACGCg -3' miRNA: 3'- aGGAUG-CggCUGCGCgACCA------AAUGCG- -5' |
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2790 | 3' | -54.3 | NC_001491.2 | + | 138145 | 0.66 | 0.958241 |
Target: 5'- gCCUcucgGCGCCuccuCGcCGCUGGagUugGCg -3' miRNA: 3'- aGGA----UGCGGcu--GC-GCGACCaaAugCG- -5' |
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2790 | 3' | -54.3 | NC_001491.2 | + | 141436 | 0.79 | 0.338228 |
Target: 5'- cCCUGCGCagGGCGCuGCUGGauuUUUACGCc -3' miRNA: 3'- aGGAUGCGg-CUGCG-CGACC---AAAUGCG- -5' |
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2790 | 3' | -54.3 | NC_001491.2 | + | 148599 | 0.67 | 0.926039 |
Target: 5'- gUCCccaucggUugGCCG-CGCGgUGGcucUGCGCg -3' miRNA: 3'- -AGG-------AugCGGCuGCGCgACCaa-AUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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