Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2790 | 3' | -54.3 | NC_001491.2 | + | 35064 | 0.66 | 0.946088 |
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Target: 5'- aCCUGUGUCGAUGuCGUaucucuaucUGGUaUACGCa -3' miRNA: 3'- aGGAUGCGGCUGC-GCG---------ACCAaAUGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 46243 | 0.66 | 0.946088 |
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Target: 5'- uUCUUGagGCgGugGCGuCUGGUUUGC-Cg -3' miRNA: 3'- -AGGAUg-CGgCugCGC-GACCAAAUGcG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 50652 | 0.66 | 0.946088 |
|
Target: 5'- gUCCUuaauaaACGCCGcgcACGCGgUcGGUUUGgGUg -3' miRNA: 3'- -AGGA------UGCGGC---UGCGCgA-CCAAAUgCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 116599 | 0.66 | 0.946088 |
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Target: 5'- cUCCUGCGCCucCGCGgC-GGg--GCGUc -3' miRNA: 3'- -AGGAUGCGGcuGCGC-GaCCaaaUGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 57386 | 0.66 | 0.945647 |
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Target: 5'- -aCUACGCUGgugggggcugagcAgGgGCUGGcUUGCGCg -3' miRNA: 3'- agGAUGCGGC-------------UgCgCGACCaAAUGCG- -5' |
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| 2790 | 3' | -54.3 | NC_001491.2 | + | 141436 | 0.79 | 0.338228 |
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Target: 5'- cCCUGCGCagGGCGCuGCUGGauuUUUACGCc -3' miRNA: 3'- aGGAUGCGg-CUGCG-CGACC---AAAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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