Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2790 | 5' | -62 | NC_001491.2 | + | 144669 | 0.66 | 0.606387 |
Target: 5'- -cGCGCUgGCUgcgCGGAugAGGgGGUCCUCGc -3' miRNA: 3'- uuCGCGG-CGA---GUCU--UCCgCCGGGAGC- -5' |
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2790 | 5' | -62 | NC_001491.2 | + | 145377 | 0.7 | 0.3536 |
Target: 5'- cGGCGCCGgagcggcagcucUUCGGGGuGGCGGCCCcagCGg -3' miRNA: 3'- uUCGCGGC------------GAGUCUU-CCGCCGGGa--GC- -5' |
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2790 | 5' | -62 | NC_001491.2 | + | 147269 | 0.66 | 0.626319 |
Target: 5'- gAGGCgGCCGcCUgAGggGaGCGGgCCUUCu -3' miRNA: 3'- -UUCG-CGGC-GAgUCuuC-CGCC-GGGAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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