Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27920 | 3' | -55.9 | NC_005886.2 | + | 39359 | 0.66 | 0.638488 |
Target: 5'- gUCgCGCAACGUCgcgGCUuccGUUGCACCa -3' miRNA: 3'- -GGaGCGUUGCGGa--CGGc--CAAUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 18597 | 0.66 | 0.638488 |
Target: 5'- -gUCGCuACGCUgcucGCCGGUcUGCugCa -3' miRNA: 3'- ggAGCGuUGCGGa---CGGCCA-AUGugGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 18281 | 0.66 | 0.616395 |
Target: 5'- gCUUGCgAACGUCUGCCGcauccauCGCCUu -3' miRNA: 3'- gGAGCG-UUGCGGACGGCcaau---GUGGA- -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 38526 | 0.66 | 0.616395 |
Target: 5'- --gUGCGGCGCg-GCCGGcaACACCa -3' miRNA: 3'- ggaGCGUUGCGgaCGGCCaaUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 13441 | 0.66 | 0.616395 |
Target: 5'- gCUCGCAACGCUgcaaGCCGug-ACGgCUg -3' miRNA: 3'- gGAGCGUUGCGGa---CGGCcaaUGUgGA- -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 43939 | 0.67 | 0.594357 |
Target: 5'- aCCggGCuucACGUCUGCCGGgca-ACCUg -3' miRNA: 3'- -GGagCGu--UGCGGACGGCCaaugUGGA- -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 44120 | 0.67 | 0.594357 |
Target: 5'- aCUUGCugcggAACGCCgGCCGcUUGCAUCg -3' miRNA: 3'- gGAGCG-----UUGCGGaCGGCcAAUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 22895 | 0.68 | 0.529281 |
Target: 5'- aCUCggGCGAUaGCgUGCCGGUgcuCACCg -3' miRNA: 3'- gGAG--CGUUG-CGgACGGCCAau-GUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 9674 | 0.69 | 0.447089 |
Target: 5'- aCCU-GCGAUGCCUGCUGcUUGCuCCg -3' miRNA: 3'- -GGAgCGUUGCGGACGGCcAAUGuGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 40853 | 0.72 | 0.330361 |
Target: 5'- gCCaUCGCGACGCCUuCCccUUGCACCUc -3' miRNA: 3'- -GG-AGCGUUGCGGAcGGccAAUGUGGA- -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 41881 | 0.74 | 0.219645 |
Target: 5'- -gUCGCAAUGCCUgcGCCGG--ACGCCg -3' miRNA: 3'- ggAGCGUUGCGGA--CGGCCaaUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 43955 | 0.75 | 0.213898 |
Target: 5'- gCCggugCGCccAUGCCUGCCGGU-GCGCCc -3' miRNA: 3'- -GGa---GCGu-UGCGGACGGCCAaUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 43919 | 0.75 | 0.213898 |
Target: 5'- gCCggugCGCccAUGCCUGCCGGU-GCGCCc -3' miRNA: 3'- -GGa---GCGu-UGCGGACGGCCAaUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 43991 | 0.75 | 0.20828 |
Target: 5'- cCCggugCGCccAUGCCUGCCGGU-GCGCCc -3' miRNA: 3'- -GGa---GCGu-UGCGGACGGCCAaUGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 16187 | 0.76 | 0.172432 |
Target: 5'- cCCUUGUAuuguacCGCCUGCCGGguuacGCGCCg -3' miRNA: 3'- -GGAGCGUu-----GCGGACGGCCaa---UGUGGa -5' |
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27920 | 3' | -55.9 | NC_005886.2 | + | 2085 | 1.09 | 0.000709 |
Target: 5'- aCCUCGCAACGCCUGCCGGUUACACCUc -3' miRNA: 3'- -GGAGCGUUGCGGACGGCCAAUGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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