Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27921 | 5' | -49.7 | NC_005886.2 | + | 25790 | 0.66 | 0.913884 |
Target: 5'- -cGCAUCGCggcCGCGugucGGCGcguuUGGUGAAUc -3' miRNA: 3'- aaCGUAGCGa--GCGC----UUGU----ACUACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 40347 | 0.66 | 0.913884 |
Target: 5'- ---gAUCGUUgGUG-ACGUGGUGAACa -3' miRNA: 3'- aacgUAGCGAgCGCuUGUACUACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 44621 | 0.66 | 0.913884 |
Target: 5'- aUUGCGUCGUUCGCuucuuUGAgucGAACg -3' miRNA: 3'- -AACGUAGCGAGCGcuuguACUa--CUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 26028 | 0.66 | 0.906954 |
Target: 5'- -gGCGUuaCGCU-GCGGGCGUGAUGucACc -3' miRNA: 3'- aaCGUA--GCGAgCGCUUGUACUACu-UG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 25846 | 0.66 | 0.892161 |
Target: 5'- -cGCGUCGaauucaagccCGCGAAUGUGAucgaUGAACa -3' miRNA: 3'- aaCGUAGCga--------GCGCUUGUACU----ACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 39171 | 0.67 | 0.867715 |
Target: 5'- -cGCGUUGCUgaaCGCacAACAUGcgGAGCg -3' miRNA: 3'- aaCGUAGCGA---GCGc-UUGUACuaCUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 6900 | 0.67 | 0.858993 |
Target: 5'- -aGCGuUCGCaUCGCGcucGACAuUGAUGAAUu -3' miRNA: 3'- aaCGU-AGCG-AGCGC---UUGU-ACUACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 9612 | 0.68 | 0.840742 |
Target: 5'- -cGCGggucauUCGCUUGCGcaaucAACGcuUGAUGAACa -3' miRNA: 3'- aaCGU------AGCGAGCGC-----UUGU--ACUACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 38281 | 0.69 | 0.769559 |
Target: 5'- gUGCGaaccgaCGUgCGCGGGCGUGAUGAGa -3' miRNA: 3'- aACGUa-----GCGaGCGCUUGUACUACUUg -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 38964 | 0.69 | 0.758631 |
Target: 5'- -cGCAUCGaagcgauggCGCGAGC--GAUGAACg -3' miRNA: 3'- aaCGUAGCga-------GCGCUUGuaCUACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 13348 | 0.69 | 0.747555 |
Target: 5'- -gGCAacgUCGCUCGCGGAUGUagcGAGCg -3' miRNA: 3'- aaCGU---AGCGAGCGCUUGUAcuaCUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 32657 | 0.69 | 0.747555 |
Target: 5'- gUUGCGUCGCUCGUu-ACGgucacaGUGAACa -3' miRNA: 3'- -AACGUAGCGAGCGcuUGUac----UACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 44087 | 0.71 | 0.631696 |
Target: 5'- -gGUAUUGCUC-CGGGCAUGAUGccGACc -3' miRNA: 3'- aaCGUAGCGAGcGCUUGUACUAC--UUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 21765 | 0.74 | 0.49325 |
Target: 5'- -cGCAUCGCaUCGCGcGGCAUcuGUGAACa -3' miRNA: 3'- aaCGUAGCG-AGCGC-UUGUAc-UACUUG- -5' |
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27921 | 5' | -49.7 | NC_005886.2 | + | 6113 | 1.07 | 0.003524 |
Target: 5'- cUUGCAUCGCUCGCGAACAUGAUGAACc -3' miRNA: 3'- -AACGUAGCGAGCGCUUGUACUACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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