Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27923 | 5' | -55.9 | NC_005886.2 | + | 41964 | 0.66 | 0.608119 |
Target: 5'- aUCGGCGCGG----GGGCGgcGuCCGg -3' miRNA: 3'- cAGCCGCGCCgaauUCUGCuuC-GGCa -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 41573 | 0.67 | 0.574943 |
Target: 5'- cGUCGGCGuCaGCgccGGACGuugcGGCCGg -3' miRNA: 3'- -CAGCCGC-GcCGaauUCUGCu---UCGGCa -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 11497 | 0.69 | 0.428892 |
Target: 5'- -cUGGCGCGGCUcgaaauGGCGAuGCUGa -3' miRNA: 3'- caGCCGCGCCGAauu---CUGCUuCGGCa -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 31040 | 0.72 | 0.284639 |
Target: 5'- uGUCGGCGUucGGCUgAAGACGGcAGCgGa -3' miRNA: 3'- -CAGCCGCG--CCGAaUUCUGCU-UCGgCa -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 33947 | 0.76 | 0.176616 |
Target: 5'- gGUCGGCGCGGCUUcgguagucGGGAUcAGGUCGg -3' miRNA: 3'- -CAGCCGCGCCGAA--------UUCUGcUUCGGCa -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 24302 | 0.76 | 0.167169 |
Target: 5'- --gGGCGCGGUgauucguGGCGAAGCCGg -3' miRNA: 3'- cagCCGCGCCGaauu---CUGCUUCGGCa -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 22115 | 0.76 | 0.162617 |
Target: 5'- uUCGGCGCGG---GAGACGAcgaaaAGCCGUu -3' miRNA: 3'- cAGCCGCGCCgaaUUCUGCU-----UCGGCA- -5' |
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27923 | 5' | -55.9 | NC_005886.2 | + | 11815 | 1.08 | 0.000814 |
Target: 5'- cGUCGGCGCGGCUUAAGACGAAGCCGUa -3' miRNA: 3'- -CAGCCGCGCCGAAUUCUGCUUCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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