miRNA: 3'- cuUCCAG--AGCGcuaGCA-ACGUGCACC- -5'
miRNA: 3'- -CUuCCAGAGcgCUAGCAA-------CGUGCA--CC- -5'
miRNA: 3'- cuUCCAGAGCGCUaGCAa--CGUGca--CC- -5'
miRNA: 3'- cuUCCAgaGC---GCUAGCAaCGUGCACC- -5'
miRNA: 3'- -CUUCCAGAGCGCUAGCAACGUGCACC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27926 | 3' | -52.2 | NC_005886.2 | + | 41863 | 0.66 | 0.858416 |
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Target: 5'- -cGGGUUgaUCGCcugCGUaUGCAUGUGGa -3' miRNA: 3'- cuUCCAG--AGCGcuaGCA-ACGUGCACC- -5' |
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| 27926 | 3' | -52.2 | NC_005886.2 | + | 45535 | 0.66 | 0.857567 |
|
Target: 5'- aGAcGGUCUCaaGAUCGUUuugaaugGCACGUucGGc -3' miRNA: 3'- -CUuCCAGAGcgCUAGCAA-------CGUGCA--CC- -5' |
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| 27926 | 3' | -52.2 | NC_005886.2 | + | 19374 | 0.66 | 0.840983 |
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Target: 5'- -cAGGUCUCGCGA-CGUgcaGCAUcuucGGg -3' miRNA: 3'- cuUCCAGAGCGCUaGCAa--CGUGca--CC- -5' |
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| 27926 | 3' | -52.2 | NC_005886.2 | + | 31583 | 0.67 | 0.80344 |
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Target: 5'- -cGGGUgaUGacaCGAUCGUaGCGCGUGGu -3' miRNA: 3'- cuUCCAgaGC---GCUAGCAaCGUGCACC- -5' |
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| 27926 | 3' | -52.2 | NC_005886.2 | + | 14998 | 1.11 | 0.001465 |
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Target: 5'- cGAAGGUCUCGCGAUCGUUGCACGUGGu -3' miRNA: 3'- -CUUCCAGAGCGCUAGCAACGUGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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