Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27926 | 5' | -49.7 | NC_005886.2 | + | 38447 | 0.66 | 0.921089 |
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Target: 5'- cGCCGCGCagcuUCGagACGGaUGAGGGAUUg -3' miRNA: 3'- aCGGUGUG----AGCg-UGCUaACUUUCUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 38579 | 0.66 | 0.933076 |
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Target: 5'- cGCCGCACUUGC-UGAUccAAGcgcGGCCg -3' miRNA: 3'- aCGGUGUGAGCGuGCUAacUUU---CUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 42454 | 0.66 | 0.938613 |
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Target: 5'- cGCCGCACUCcggguggauGCGCGGUgcggcaACCu -3' miRNA: 3'- aCGGUGUGAG---------CGUGCUAacuuucUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 42592 | 0.69 | 0.795003 |
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Target: 5'- aGCCGCGCgcauaUCGaCGCGAU----GGACCg -3' miRNA: 3'- aCGGUGUG-----AGC-GUGCUAacuuUCUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 44124 | 0.67 | 0.907883 |
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Target: 5'- cGCCGCaACUCGUGcCGAcgcaGAAAGcgGCCg -3' miRNA: 3'- aCGGUG-UGAGCGU-GCUaa--CUUUC--UGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 45622 | 0.69 | 0.815035 |
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Target: 5'- aGUCACgcuuacgggucaACUCGCGCuGUUGAuguugaucgAAGACCu -3' miRNA: 3'- aCGGUG------------UGAGCGUGcUAACU---------UUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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