Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27927 | 5' | -53 | NC_005886.2 | + | 21987 | 0.66 | 0.803734 |
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Target: 5'- uCGCGUGAUCAcgaaucgccaucaUCGUCUGAUgGACa- -3' miRNA: 3'- -GCGCGCUAGUa------------AGUAGGCUGgCUGcu -5' |
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| 27927 | 5' | -53 | NC_005886.2 | + | 21814 | 0.66 | 0.807591 |
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Target: 5'- gCGUaGCGAUCAgggcgcaCAUCUGAugcCCGAUGAa -3' miRNA: 3'- -GCG-CGCUAGUaa-----GUAGGCU---GGCUGCU- -5' |
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| 27927 | 5' | -53 | NC_005886.2 | + | 28067 | 0.66 | 0.807591 |
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Target: 5'- aGCGCGAUCGUggaaagGUCCG-CaCGGCa- -3' miRNA: 3'- gCGCGCUAGUAag----UAGGCuG-GCUGcu -5' |
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| 27927 | 5' | -53 | NC_005886.2 | + | 15072 | 0.66 | 0.817101 |
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Target: 5'- cCGCGCGAUgGUgcgccgaauUCAUguaUCGACgCGACGu -3' miRNA: 3'- -GCGCGCUAgUA---------AGUA---GGCUG-GCUGCu -5' |
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| 27927 | 5' | -53 | NC_005886.2 | + | 46956 | 0.74 | 0.378749 |
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Target: 5'- -aUGCGAUCAUUCGcauugccgaUCUGGCCGGCGc -3' miRNA: 3'- gcGCGCUAGUAAGU---------AGGCUGGCUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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