Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27928 | 5' | -55.2 | NC_005886.2 | + | 13440 | 0.66 | 0.704147 |
Target: 5'- -cUCGCAACgCUgcaaGCCGUgAcGGCUGGUGc -3' miRNA: 3'- caAGCGUUG-GA----UGGCAgU-CUGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 42521 | 0.66 | 0.682284 |
Target: 5'- cGUUUGCggUCUACuCGaUCAcAUCGGCGu -3' miRNA: 3'- -CAAGCGuuGGAUG-GC-AGUcUGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 2717 | 0.66 | 0.682284 |
Target: 5'- --gCGCGGCCgGCgUGUCAGuugcaGCCGGUGu -3' miRNA: 3'- caaGCGUUGGaUG-GCAGUC-----UGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 13406 | 0.66 | 0.671274 |
Target: 5'- cUUCGCAccACCauagAUCGcaacCGGACCGGCu -3' miRNA: 3'- cAAGCGU--UGGa---UGGCa---GUCUGGCCGc -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 22659 | 0.67 | 0.649152 |
Target: 5'- cGUUCGCAcg--GCaGUCGGACgGGCGu -3' miRNA: 3'- -CAAGCGUuggaUGgCAGUCUGgCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 29844 | 0.67 | 0.615875 |
Target: 5'- --cCGCAACCUACCGaccUCGGGggcuaUGGCu -3' miRNA: 3'- caaGCGUUGGAUGGC---AGUCUg----GCCGc -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 29634 | 0.67 | 0.593746 |
Target: 5'- uUUCGUGACCUucacacGCuCGaUCAG-CCGGCGu -3' miRNA: 3'- cAAGCGUUGGA------UG-GC-AGUCuGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 21318 | 0.68 | 0.560821 |
Target: 5'- -cUCGCGaugACCaaaCGUCAGACCGuGCu -3' miRNA: 3'- caAGCGU---UGGaugGCAGUCUGGC-CGc -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 44126 | 0.68 | 0.549951 |
Target: 5'- -gUUGCAACUUGCUG-CGGaacGCCGGCc -3' miRNA: 3'- caAGCGUUGGAUGGCaGUC---UGGCCGc -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 18224 | 0.68 | 0.539148 |
Target: 5'- --aUGCGuCUUGCaCGgguugCAGACCGGCGa -3' miRNA: 3'- caaGCGUuGGAUG-GCa----GUCUGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 23402 | 0.69 | 0.516706 |
Target: 5'- aUUCGCAuggaacugcaccaGCCgcucuacaaACCGUCGacGACCGGCa -3' miRNA: 3'- cAAGCGU-------------UGGa--------UGGCAGU--CUGGCCGc -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 46964 | 0.71 | 0.407495 |
Target: 5'- aUUCGCA--UUGCCGaUCuGGCCGGCGc -3' miRNA: 3'- cAAGCGUugGAUGGC-AGuCUGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 41263 | 0.71 | 0.380049 |
Target: 5'- aGUUCGUcgUCgcacACUGUCAGcACCGGCGc -3' miRNA: 3'- -CAAGCGuuGGa---UGGCAGUC-UGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 18513 | 0.71 | 0.380049 |
Target: 5'- cUUCGCGAaUUGCag-CAGACCGGCGa -3' miRNA: 3'- cAAGCGUUgGAUGgcaGUCUGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 11610 | 0.71 | 0.37118 |
Target: 5'- cUUCGCGugCaACCGUC--GCCGGCa -3' miRNA: 3'- cAAGCGUugGaUGGCAGucUGGCCGc -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 38905 | 0.72 | 0.345429 |
Target: 5'- -aUCGCuucgauGCgCUGCCGUCGucGAUCGGCGc -3' miRNA: 3'- caAGCGu-----UG-GAUGGCAGU--CUGGCCGC- -5' |
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27928 | 5' | -55.2 | NC_005886.2 | + | 16290 | 1.08 | 0.001016 |
Target: 5'- cGUUCGCAACCUACCGUCAGACCGGCGc -3' miRNA: 3'- -CAAGCGUUGGAUGGCAGUCUGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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