Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27929 | 3' | -52.6 | NC_005886.2 | + | 17781 | 0.66 | 0.845221 |
Target: 5'- aGGCgca---GGCGGUaCGGG-CGUGCu -3' miRNA: 3'- -CCGauauugCUGCCGaGCCCaGCAUG- -5' |
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27929 | 3' | -52.6 | NC_005886.2 | + | 36191 | 0.66 | 0.836284 |
Target: 5'- cGGCaucGGCGGCGGCaCGGGcggcaUCGgcgGCg -3' miRNA: 3'- -CCGauaUUGCUGCCGaGCCC-----AGCa--UG- -5' |
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27929 | 3' | -52.6 | NC_005886.2 | + | 26287 | 0.67 | 0.778319 |
Target: 5'- uGCUGc-ACGAUGcGCUCGauucugucgaGGUCGUGCu -3' miRNA: 3'- cCGAUauUGCUGC-CGAGC----------CCAGCAUG- -5' |
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27929 | 3' | -52.6 | NC_005886.2 | + | 36242 | 0.68 | 0.714692 |
Target: 5'- cGGCaucGGCGGCGGCaCGGG-CGgcgGCg -3' miRNA: 3'- -CCGauaUUGCUGCCGaGCCCaGCa--UG- -5' |
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27929 | 3' | -52.6 | NC_005886.2 | + | 36359 | 0.68 | 0.714692 |
Target: 5'- cGGCaucGGCGGCGGCaCGGG-CGgcgGCa -3' miRNA: 3'- -CCGauaUUGCUGCCGaGCCCaGCa--UG- -5' |
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27929 | 3' | -52.6 | NC_005886.2 | + | 37007 | 0.68 | 0.702617 |
Target: 5'- aGCUGauccGCGACGGUcucgaccuuggcaUCGGGgaaggCGUACg -3' miRNA: 3'- cCGAUau--UGCUGCCG-------------AGCCCa----GCAUG- -5' |
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27929 | 3' | -52.6 | NC_005886.2 | + | 16807 | 1.13 | 0.000871 |
Target: 5'- cGGCUAUAACGACGGCUCGGGUCGUACg -3' miRNA: 3'- -CCGAUAUUGCUGCCGAGCCCAGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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