Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27929 | 5' | -55.3 | NC_005886.2 | + | 22573 | 0.66 | 0.666081 |
Target: 5'- aCUGAGCGcgagcacacgcccGUCCGA--CUGCCGuGCGa- -3' miRNA: 3'- -GACUUGC-------------UAGGCUugGACGGC-CGCau -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 12627 | 0.66 | 0.65607 |
Target: 5'- aUGGGCGGUCCGA-CCgauCCGcGCGa- -3' miRNA: 3'- gACUUGCUAGGCUuGGac-GGC-CGCau -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 34906 | 0.67 | 0.589226 |
Target: 5'- aCUGcAACgGGUCCGAACCgcccgaaauUGCCGGUc-- -3' miRNA: 3'- -GAC-UUG-CUAGGCUUGG---------ACGGCCGcau -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 19567 | 0.68 | 0.513004 |
Target: 5'- uUGAACG-UCCG-GCC-GCUGGCGUu -3' miRNA: 3'- gACUUGCuAGGCuUGGaCGGCCGCAu -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 41747 | 0.71 | 0.384321 |
Target: 5'- -gGAACcAUUCGAgGCCUGCCGGCa-- -3' miRNA: 3'- gaCUUGcUAGGCU-UGGACGGCCGcau -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 2734 | 0.71 | 0.356927 |
Target: 5'- --aAACGGUUCGAAUCUucgcgcgGCCGGCGUGu -3' miRNA: 3'- gacUUGCUAGGCUUGGA-------CGGCCGCAU- -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 40010 | 0.71 | 0.348388 |
Target: 5'- aUGAACGAUUCGGuaggucgggccauGCUgaUGCCGGCGa- -3' miRNA: 3'- gACUUGCUAGGCU-------------UGG--ACGGCCGCau -5' |
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27929 | 5' | -55.3 | NC_005886.2 | + | 16771 | 1.07 | 0.001194 |
Target: 5'- cCUGAACGAUCCGAACCUGCCGGCGUAc -3' miRNA: 3'- -GACUUGCUAGGCUUGGACGGCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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